大泉洋

Professor Layton (voice)

堀北真希

Luke Triton (voice)

相武紗季

Emmy Altava (voice)

Nana Mizuki

Janice Quatlane (voice)

渡部篤郎

Jean Descole (voice)

Hochu Otsuka

Inspector Clamp Grosky (voice)

家弓家正

Oswald Whistler (voice)

Fumiko Orikasa

Melina Whistler (voice)

井上喜久子

Celia Raidley (voice)

諸星すみれ

Nina (voice)

飯塚昭三

Curtis O'Donnell (voice)

Kenta Miyake

Marco Brock (voice)

豊口めぐみ

Amelia Ruth (voice)

中田譲治

Frederick Bargland (voice)

LiLiCo

Annie Dretche (voice)

橋本昌和