Neil Patrick Harris

Peter Parker / Spider-Man (voice)

Lisa Loeb

Mary Jane Watson (voice)

Ian Ziering

Harry Osborn (voice)

Keith Carradine

J. Jonah Jameson (voice)

Ed Asner

Officer Barr (voice)

Rino Romano

Muang / Drip Den guy (voice)

Ethan Embry

Max Dillon / Electro (voice)

Julie Nathanson

Sally Johnson (voice)

Virginia Madsen

Silver Sable (voice)

Michael Dorn

Kraven the Hunter (voice)

James Marsters

Sergei (voice)

Clancy Brown

Raymond (voice)

Jeffrey Combs

Professor (voice)

Karen Maruyama

Head Protestor (voice)

Jeremy Piven

Roland Gaines (voice)

Morgan Gendel

Marsha Griffin

Brian Michael Bendis